Construct: shRNA TRCN0000174933
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_172739.1-617s1c1
- DNA Barcode:
- GAAAGAAACCACAGCAAGAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Arhgap35 (232906)
- Gene Description:
- Rho GTPase activating protein 35
- Transcript:
- RefSeq NM_172739.1 (NON-CURRENT)
- Match location:
- Position 617 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000174933)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAAGAAACCACAGCAAGAAA
- Hairpin Sequence:
-
5'-CCGG-GAAAGAAACCACAGCAAGAAA-CTCGAG-TTTCTTGCTGTGGTTTCTTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAAGAAACCACAGCAAGAAACTCGAGTTTCTTGCTGTGGTTTCTTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAAGAAACCACAGCAAGAAACTCGAGTTTCTTGCTGTGGTTTCTTTC-3'
Other clones with same target sequence:
(none)
