Construct: shRNA TRCN0000175059

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198898.1-302s1c1
DNA Barcode:
GAAGTTCATGACAGTGATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ssxb3 (278174)
Gene Description:
synovial sarcoma, X member B3
Transcript:
RefSeq NM_198898.1 (NON-CURRENT)
Match location:
Position 302 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175059)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTTCATGACAGTGATGAT
Hairpin Sequence:
5'-CCGG-GAAGTTCATGACAGTGATGAT-CTCGAG-ATCATCACTGTCATGAACTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTTCATGACAGTGATGATCTCGAGATCATCACTGTCATGAACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTTCATGACAGTGATGATCTCGAGATCATCACTGTCATGAACTTC-3'

Other clones with same target sequence:

(none)