Construct: shRNA TRCN0000175065

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023565.1-1950s1c1
DNA Barcode:
GAAGCAATATGCTTGTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cse1l (110750)
Gene Description:
chromosome segregation 1-like (S. cerevisiae)
Transcript:
RefSeq NM_023565.1 (NON-CURRENT)
Match location:
Position 1950 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175065)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCAATATGCTTGTCTATA
Hairpin Sequence:
5'-CCGG-GAAGCAATATGCTTGTCTATA-CTCGAG-TATAGACAAGCATATTGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCAATATGCTTGTCTATACTCGAGTATAGACAAGCATATTGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCAATATGCTTGTCTATACTCGAGTATAGACAAGCATATTGCTTC-3'

Other clones with same target sequence:

  1. TRCN0000314419