Construct: shRNA TRCN0000175083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177734.2-715s1c1
DNA Barcode:
GTTTGCTATACTCAACTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrd1 (243277)
Gene Description:
adhesion G protein-coupled receptor D1
Transcript:
RefSeq NM_177734.2 (NON-CURRENT)
Match location:
Position 715 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGCTATACTCAACTCTTT
Hairpin Sequence:
5'-CCGG-GTTTGCTATACTCAACTCTTT-CTCGAG-AAAGAGTTGAGTATAGCAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGCTATACTCAACTCTTTCTCGAGAAAGAGTTGAGTATAGCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGCTATACTCAACTCTTTCTCGAGAAAGAGTTGAGTATAGCAAAC-3'

Other clones with same target sequence:

(none)