Construct: shRNA TRCN0000175133
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_178642.3-1747s1c1
- DNA Barcode:
- CGGAGTTATCATCTATAGAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ano1 (101772)
- Gene Description:
- anoctamin 1, calcium activated chloride channel
- Transcript:
- RefSeq NM_178642.3 (NON-CURRENT)
- Match location:
- Position 1747 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGGAGTTATCATCTATAGAAT
- Hairpin Sequence:
-
5'-CCGG-CGGAGTTATCATCTATAGAAT-CTCGAG-ATTCTATAGATGATAACTCCG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGAGTTATCATCTATAGAATCTCGAGATTCTATAGATGATAACTCCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGAGTTATCATCTATAGAATCTCGAGATTCTATAGATGATAACTCCG-3'
Other clones with same target sequence:
(none)
