Construct: shRNA TRCN0000175134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178714.2-1721s1c1
DNA Barcode:
CGGAATCCGTATGTTTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrfn5 (238205)
Gene Description:
leucine rich repeat and fibronectin type III domain containing 5
Transcript:
RefSeq NM_178714.2 (NON-CURRENT)
Match location:
Position 1721 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAATCCGTATGTTTCAAAT
Hairpin Sequence:
5'-CCGG-CGGAATCCGTATGTTTCAAAT-CTCGAG-ATTTGAAACATACGGATTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAATCCGTATGTTTCAAATCTCGAGATTTGAAACATACGGATTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAATCCGTATGTTTCAAATCTCGAGATTTGAAACATACGGATTCCG-3'

Other clones with same target sequence:

(none)