Construct: shRNA TRCN0000175169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178701.2-2940s1c1
DNA Barcode:
GCATTGAATCAAGATGTCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc8d (231549)
Gene Description:
leucine rich repeat containing 8D
Transcript:
RefSeq NM_178701.2 (NON-CURRENT)
Match location:
Position 2940 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTGAATCAAGATGTCAAT
Hairpin Sequence:
5'-CCGG-GCATTGAATCAAGATGTCAAT-CTCGAG-ATTGACATCTTGATTCAATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTGAATCAAGATGTCAATCTCGAGATTGACATCTTGATTCAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTGAATCAAGATGTCAATCTCGAGATTGACATCTTGATTCAATGC-3'

Other clones with same target sequence:

(none)