Construct: shRNA TRCN0000175211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153803.1-3194s1c1
DNA Barcode:
CCAGAACATATCATTGAGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glb1l2 (244757)
Gene Description:
galactosidase, beta 1-like 2
Transcript:
RefSeq NM_153803.1 (CURRENT)
Match location:
Position 3194 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAACATATCATTGAGCTA
Hairpin Sequence:
5'-CCGG-CCAGAACATATCATTGAGCTA-CTCGAG-TAGCTCAATGATATGTTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAACATATCATTGAGCTACTCGAGTAGCTCAATGATATGTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAACATATCATTGAGCTACTCGAGTAGCTCAATGATATGTTCTGG-3'

Other clones with same target sequence:

(none)