Construct: shRNA TRCN0000175222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021512.1-4066s1c1
DNA Barcode:
CGGCAAATTGAAATTCTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nup160 (59015)
Gene Description:
nucleoporin 160
Transcript:
RefSeq NM_021512.1 (NON-CURRENT)
Match location:
Position 4066 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAAATTGAAATTCTGGAA
Hairpin Sequence:
5'-CCGG-CGGCAAATTGAAATTCTGGAA-CTCGAG-TTCCAGAATTTCAATTTGCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAAATTGAAATTCTGGAACTCGAGTTCCAGAATTTCAATTTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAAATTGAAATTCTGGAACTCGAGTTCCAGAATTTCAATTTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000340603