Construct: shRNA TRCN0000175237

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175547.2-469s1c1
DNA Barcode:
CAGCTACAGAAGAACTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nlrc3 (268857)
Gene Description:
NLR family, CARD domain containing 3
Transcript:
RefSeq NM_175547.2 (NON-CURRENT)
Match location:
Position 469 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175237)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTACAGAAGAACTTAATT
Hairpin Sequence:
5'-CCGG-CAGCTACAGAAGAACTTAATT-CTCGAG-AATTAAGTTCTTCTGTAGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTACAGAAGAACTTAATTCTCGAGAATTAAGTTCTTCTGTAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTACAGAAGAACTTAATTCTCGAGAATTAAGTTCTTCTGTAGCTG-3'

Other clones with same target sequence:

(none)