Construct: shRNA TRCN0000175288

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026521.3-375s1c1
DNA Barcode:
CCAAAGCTGCCTTAATATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp706 (68036)
Gene Description:
zinc finger protein 706
Transcript:
RefSeq NM_026521.3 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175288)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCTGCCTTAATATATA
Hairpin Sequence:
5'-CCGG-CCAAAGCTGCCTTAATATATA-CTCGAG-TATATATTAAGGCAGCTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCTGCCTTAATATATACTCGAGTATATATTAAGGCAGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCTGCCTTAATATATACTCGAGTATATATTAAGGCAGCTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000285884