Construct: shRNA TRCN0000175315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026437.1-1867s1c1
DNA Barcode:
CCAGCCCATAATGCTTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam45a (67894)
Gene Description:
family with sequence similarity 45, member A
Transcript:
RefSeq NM_026437.1 (NON-CURRENT)
Match location:
Position 1867 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCCCATAATGCTTTCATA
Hairpin Sequence:
5'-CCGG-CCAGCCCATAATGCTTTCATA-CTCGAG-TATGAAAGCATTATGGGCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCCCATAATGCTTTCATACTCGAGTATGAAAGCATTATGGGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCCCATAATGCTTTCATACTCGAGTATGAAAGCATTATGGGCTGG-3'

Other clones with same target sequence:

(none)