Construct: shRNA TRCN0000175456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025759.1-651s1c1
DNA Barcode:
CCAGAATATCAGGTCTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Speer4d (360220)
Gene Description:
spermatogenesis associated glutamate (E)-rich protein 4D
Transcript:
RefSeq NM_025759.1 (NON-CURRENT)
Match location:
Position 651 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAATATCAGGTCTCAGAA
Hairpin Sequence:
5'-CCGG-CCAGAATATCAGGTCTCAGAA-CTCGAG-TTCTGAGACCTGATATTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAATATCAGGTCTCAGAACTCGAGTTCTGAGACCTGATATTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAATATCAGGTCTCAGAACTCGAGTTCTGAGACCTGATATTCTGG-3'

Other clones with same target sequence:

(none)