Construct: shRNA TRCN0000175585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025455.1-651s1c1
DNA Barcode:
CAATTCCATACAGAAGCTACA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc28b (66264)
Gene Description:
coiled coil domain containing 28B
Transcript:
RefSeq NM_025455.1 (NON-CURRENT)
Match location:
Position 651 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATTCCATACAGAAGCTACA
Hairpin Sequence:
5'-CCGG-CAATTCCATACAGAAGCTACA-CTCGAG-TGTAGCTTCTGTATGGAATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATTCCATACAGAAGCTACACTCGAGTGTAGCTTCTGTATGGAATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATTCCATACAGAAGCTACACTCGAGTGTAGCTTCTGTATGGAATTG-3'

Other clones with same target sequence:

(none)