Construct: shRNA TRCN0000175590

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027973.2-1552s1c1
DNA Barcode:
CGTCTGCTGAAATATGTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cenpu (71876)
Gene Description:
centromere protein U
Transcript:
RefSeq NM_027973.2 (NON-CURRENT)
Match location:
Position 1552 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175590)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGCTGAAATATGTCAAA
Hairpin Sequence:
5'-CCGG-CGTCTGCTGAAATATGTCAAA-CTCGAG-TTTGACATATTTCAGCAGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGCTGAAATATGTCAAACTCGAGTTTGACATATTTCAGCAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGCTGAAATATGTCAAACTCGAGTTTGACATATTTCAGCAGACG-3'

Other clones with same target sequence:

(none)