Construct: shRNA TRCN0000175742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177474.2-650s1c1
DNA Barcode:
GCGACTTGGTTGAATTAAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pum3 (52874)
Gene Description:
pumilio RNA-binding family member 3
Transcript:
RefSeq NM_177474.2 (NON-CURRENT)
Match location:
Position 650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGACTTGGTTGAATTAAGTA
Hairpin Sequence:
5'-CCGG-GCGACTTGGTTGAATTAAGTA-CTCGAG-TACTTAATTCAACCAAGTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGACTTGGTTGAATTAAGTACTCGAGTACTTAATTCAACCAAGTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGACTTGGTTGAATTAAGTACTCGAGTACTTAATTCAACCAAGTCGC-3'

Other clones with same target sequence:

(none)