Construct: shRNA TRCN0000175769

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177388.2-1046s1c1
DNA Barcode:
GTATTACCTCAGCCATTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc41a2 (338365)
Gene Description:
solute carrier family 41, member 2
Transcript:
RefSeq NM_177388.2 (NON-CURRENT)
Match location:
Position 1046 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175769)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATTACCTCAGCCATTCCAT
Hairpin Sequence:
5'-CCGG-GTATTACCTCAGCCATTCCAT-CTCGAG-ATGGAATGGCTGAGGTAATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATTACCTCAGCCATTCCATCTCGAGATGGAATGGCTGAGGTAATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATTACCTCAGCCATTCCATCTCGAGATGGAATGGCTGAGGTAATAC-3'

Other clones with same target sequence:

  1. TRCN0000353343