Construct: shRNA TRCN0000175781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018872.1-5689s1c1
DNA Barcode:
GCTGTTGTGCTTAAAGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem131 (56030)
Gene Description:
transmembrane protein 131
Transcript:
RefSeq NM_018872.1 (NON-CURRENT)
Match location:
Position 5689 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGTGCTTAAAGACAAA
Hairpin Sequence:
5'-CCGG-GCTGTTGTGCTTAAAGACAAA-CTCGAG-TTTGTCTTTAAGCACAACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGTGCTTAAAGACAAACTCGAGTTTGTCTTTAAGCACAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGTGCTTAAAGACAAACTCGAGTTTGTCTTTAAGCACAACAGC-3'

Other clones with same target sequence:

(none)