Construct: shRNA TRCN0000175787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026040.2-224s1c1
DNA Barcode:
GCCATGAAGGAATTGAAACCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srfbp1 (67222)
Gene Description:
serum response factor binding protein 1
Transcript:
RefSeq NM_026040.2 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGAAGGAATTGAAACCT
Hairpin Sequence:
5'-CCGG-GCCATGAAGGAATTGAAACCT-CTCGAG-AGGTTTCAATTCCTTCATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGAAGGAATTGAAACCTCTCGAGAGGTTTCAATTCCTTCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGAAGGAATTGAAACCTCTCGAGAGGTTTCAATTCCTTCATGGC-3'

Other clones with same target sequence:

(none)