Construct: shRNA TRCN0000175815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025797.1-150s1c1
DNA Barcode:
GATCCTGCATCATAAGGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyb5a (109672)
Gene Description:
cytochrome b5 type A (microsomal)
Transcript:
RefSeq NM_025797.1 (NON-CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCTGCATCATAAGGTGTA
Hairpin Sequence:
5'-CCGG-GATCCTGCATCATAAGGTGTA-CTCGAG-TACACCTTATGATGCAGGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCTGCATCATAAGGTGTACTCGAGTACACCTTATGATGCAGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCTGCATCATAAGGTGTACTCGAGTACACCTTATGATGCAGGATC-3'

Other clones with same target sequence:

  1. TRCN0000292805