Construct: shRNA TRCN0000175819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026776.3-368s1c1
DNA Barcode:
GCCAGAATAACTCTGTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps25 (28084)
Gene Description:
vacuolar protein sorting 25
Transcript:
RefSeq NM_026776.3 (NON-CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGAATAACTCTGTGTTTA
Hairpin Sequence:
5'-CCGG-GCCAGAATAACTCTGTGTTTA-CTCGAG-TAAACACAGAGTTATTCTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGAATAACTCTGTGTTTACTCGAGTAAACACAGAGTTATTCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGAATAACTCTGTGTTTACTCGAGTAAACACAGAGTTATTCTGGC-3'

Other clones with same target sequence:

  1. TRCN0000336110