Construct: shRNA TRCN0000175911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011412.1-2582s1c1
DNA Barcode:
GCTCCTTCAATGATCTGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slit3 (20564)
Gene Description:
slit homolog 3 (Drosophila)
Transcript:
RefSeq NM_011412.1 (NON-CURRENT)
Match location:
Position 2582 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCTTCAATGATCTGACAT
Hairpin Sequence:
5'-CCGG-GCTCCTTCAATGATCTGACAT-CTCGAG-ATGTCAGATCATTGAAGGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCTTCAATGATCTGACATCTCGAGATGTCAGATCATTGAAGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCTTCAATGATCTGACATCTCGAGATGTCAGATCATTGAAGGAGC-3'

Other clones with same target sequence:

(none)