Construct: shRNA TRCN0000175951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026333.2-451s1c1
DNA Barcode:
GAGAAGAGTATGTCAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010106E10Rik (67715)
Gene Description:
RIKEN cDNA 2010106E10 gene
Transcript:
RefSeq NM_026333.2 (NON-CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGAGTATGTCAGAACTT
Hairpin Sequence:
5'-CCGG-GAGAAGAGTATGTCAGAACTT-CTCGAG-AAGTTCTGACATACTCTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGAGTATGTCAGAACTTCTCGAGAAGTTCTGACATACTCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGAGTATGTCAGAACTTCTCGAGAAGTTCTGACATACTCTTCTC-3'

Other clones with same target sequence:

(none)