Construct: shRNA TRCN0000175981

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080638.1-1263s1c1
DNA Barcode:
GCATCTATGTTCAGGATGTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mvp (78388)
Gene Description:
major vault protein
Transcript:
RefSeq NM_080638.1 (NON-CURRENT)
Match location:
Position 1263 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175981)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCTATGTTCAGGATGTCA
Hairpin Sequence:
5'-CCGG-GCATCTATGTTCAGGATGTCA-CTCGAG-TGACATCCTGAACATAGATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCTATGTTCAGGATGTCACTCGAGTGACATCCTGAACATAGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCTATGTTCAGGATGTCACTCGAGTGACATCCTGAACATAGATGC-3'

Other clones with same target sequence:

  1. TRCN0000292855