Construct: shRNA TRCN0000175984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144541.1-299s1c1
DNA Barcode:
GATTTCATCTTTGGAGAGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Babam2 (107976)
Gene Description:
BRISC and BRCA1 A complex member 2
Transcript:
RefSeq NM_144541.1 (CURRENT)
Match location:
Position 299 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000175984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTCATCTTTGGAGAGGAT
Hairpin Sequence:
5'-CCGG-GATTTCATCTTTGGAGAGGAT-CTCGAG-ATCCTCTCCAAAGATGAAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTCATCTTTGGAGAGGATCTCGAGATCCTCTCCAAAGATGAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTCATCTTTGGAGAGGATCTCGAGATCCTCTCCAAAGATGAAATC-3'

Other clones with same target sequence:

(none)