Construct: shRNA TRCN0000176090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023743.1-3108s1c1
DNA Barcode:
GCACACTATGTGAAGATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif4enif1 (74203)
Gene Description:
eukaryotic translation initiation factor 4E nuclear import factor 1
Transcript:
RefSeq NM_023743.1 (NON-CURRENT)
Match location:
Position 3108 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACACTATGTGAAGATGTTT
Hairpin Sequence:
5'-CCGG-GCACACTATGTGAAGATGTTT-CTCGAG-AAACATCTTCACATAGTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACACTATGTGAAGATGTTTCTCGAGAAACATCTTCACATAGTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACACTATGTGAAGATGTTTCTCGAGAAACATCTTCACATAGTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000340295