Construct: shRNA TRCN0000176096
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027434.1-1567s1c1
- DNA Barcode:
- GCCGACATTTCCTGATCTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Rprd1b (70470)
- Gene Description:
- regulation of nuclear pre-mRNA domain containing 1B
- Transcript:
- RefSeq NM_027434.1 (NON-CURRENT)
- Match location:
- Position 1567 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000176096)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCGACATTTCCTGATCTAAA
- Hairpin Sequence:
-
5'-CCGG-GCCGACATTTCCTGATCTAAA-CTCGAG-TTTAGATCAGGAAATGTCGGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCGACATTTCCTGATCTAAACTCGAGTTTAGATCAGGAAATGTCGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCGACATTTCCTGATCTAAACTCGAGTTTAGATCAGGAAATGTCGGC-3'
Other clones with same target sequence:
-
TRCN0000241220
