Construct: shRNA TRCN0000176285

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028850.1-703s1c1
DNA Barcode:
GTTATACCACAAGCTGTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chic2 (74277)
Gene Description:
cysteine-rich hydrophobic domain 2
Transcript:
RefSeq NM_028850.1 (NON-CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176285)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTATACCACAAGCTGTGCTT
Hairpin Sequence:
5'-CCGG-GTTATACCACAAGCTGTGCTT-CTCGAG-AAGCACAGCTTGTGGTATAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTATACCACAAGCTGTGCTTCTCGAGAAGCACAGCTTGTGGTATAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTATACCACAAGCTGTGCTTCTCGAGAAGCACAGCTTGTGGTATAAC-3'

Other clones with same target sequence:

  1. TRCN0000350150