Construct: shRNA TRCN0000176311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172257.1-3866s1c1
DNA Barcode:
CGTCCAGTATTTAGCCAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sidt2 (214597)
Gene Description:
SID1 transmembrane family, member 2
Transcript:
RefSeq NM_172257.1 (NON-CURRENT)
Match location:
Position 3866 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCCAGTATTTAGCCAAGTT
Hairpin Sequence:
5'-CCGG-CGTCCAGTATTTAGCCAAGTT-CTCGAG-AACTTGGCTAAATACTGGACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCCAGTATTTAGCCAAGTTCTCGAGAACTTGGCTAAATACTGGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCCAGTATTTAGCCAAGTTCTCGAGAACTTGGCTAAATACTGGACG-3'

Other clones with same target sequence:

  1. TRCN0000292684