Construct: shRNA TRCN0000176313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175188.3-2869s1c1
DNA Barcode:
CGAAAGAAAGATGACCACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
March1 (72925)
Gene Description:
membrane-associated ring finger (C3HC4) 1
Transcript:
RefSeq NM_175188.3 (NON-CURRENT)
Match location:
Position 2869 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGAAAGATGACCACATT
Hairpin Sequence:
5'-CCGG-CGAAAGAAAGATGACCACATT-CTCGAG-AATGTGGTCATCTTTCTTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGAAAGATGACCACATTCTCGAGAATGTGGTCATCTTTCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGAAAGATGACCACATTCTCGAGAATGTGGTCATCTTTCTTTCG-3'

Other clones with same target sequence:

(none)