Construct: shRNA TRCN0000176331

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054041.1-1394s1c1
DNA Barcode:
CCACAGTAGATGCCTCTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Antxr1 (69538)
Gene Description:
anthrax toxin receptor 1
Transcript:
RefSeq NM_054041.1 (NON-CURRENT)
Match location:
Position 1394 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176331)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTAGATGCCTCTTATT
Hairpin Sequence:
5'-CCGG-CCACAGTAGATGCCTCTTATT-CTCGAG-AATAAGAGGCATCTACTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTAGATGCCTCTTATTCTCGAGAATAAGAGGCATCTACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTAGATGCCTCTTATTCTCGAGAATAAGAGGCATCTACTGTGG-3'

Other clones with same target sequence:

(none)