Construct: shRNA TRCN0000176359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172475.2-2239s1c1
DNA Barcode:
CCCACTATGTTCATTCCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frmd4a (209630)
Gene Description:
FERM domain containing 4A
Transcript:
RefSeq NM_172475.2 (NON-CURRENT)
Match location:
Position 2239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTATGTTCATTCCACAA
Hairpin Sequence:
5'-CCGG-CCCACTATGTTCATTCCACAA-CTCGAG-TTGTGGAATGAACATAGTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTATGTTCATTCCACAACTCGAGTTGTGGAATGAACATAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTATGTTCATTCCACAACTCGAGTTGTGGAATGAACATAGTGGG-3'

Other clones with same target sequence:

(none)