Construct: shRNA TRCN0000176432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026644.1-492s1c1
DNA Barcode:
CCTCAATCACAAGTTTGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agpat4 (68262)
Gene Description:
1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
Transcript:
RefSeq NM_026644.1 (NON-CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAATCACAAGTTTGAGAT
Hairpin Sequence:
5'-CCGG-CCTCAATCACAAGTTTGAGAT-CTCGAG-ATCTCAAACTTGTGATTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAATCACAAGTTTGAGATCTCGAGATCTCAAACTTGTGATTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAATCACAAGTTTGAGATCTCGAGATCTCAAACTTGTGATTGAGG-3'

Other clones with same target sequence:

(none)