Construct: shRNA TRCN0000176551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178398.2-1720s1c1
DNA Barcode:
CCTGCTTATGAATTTAGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wipi2 (74781)
Gene Description:
WD repeat domain, phosphoinositide interacting 2
Transcript:
RefSeq NM_178398.2 (NON-CURRENT)
Match location:
Position 1720 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTTATGAATTTAGCTTT
Hairpin Sequence:
5'-CCGG-CCTGCTTATGAATTTAGCTTT-CTCGAG-AAAGCTAAATTCATAAGCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTTATGAATTTAGCTTTCTCGAGAAAGCTAAATTCATAAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTTATGAATTTAGCTTTCTCGAGAAAGCTAAATTCATAAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000319895