Construct: shRNA TRCN0000176611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175178.2-843s1c1
DNA Barcode:
CATAAGTTCCAGGTGAAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aifm3 (72168)
Gene Description:
apoptosis-inducing factor, mitochondrion-associated 3
Transcript:
RefSeq NM_175178.2 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAAGTTCCAGGTGAAGATT
Hairpin Sequence:
5'-CCGG-CATAAGTTCCAGGTGAAGATT-CTCGAG-AATCTTCACCTGGAACTTATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAAGTTCCAGGTGAAGATTCTCGAGAATCTTCACCTGGAACTTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAAGTTCCAGGTGAAGATTCTCGAGAATCTTCACCTGGAACTTATG-3'

Other clones with same target sequence:

(none)