Construct: shRNA TRCN0000176637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080446.1-4288s1c1
DNA Barcode:
CCTGATCCTAAACATAACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Helb (117599)
Gene Description:
helicase (DNA) B
Transcript:
RefSeq NM_080446.1 (NON-CURRENT)
Match location:
Position 4288 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATCCTAAACATAACTTT
Hairpin Sequence:
5'-CCGG-CCTGATCCTAAACATAACTTT-CTCGAG-AAAGTTATGTTTAGGATCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATCCTAAACATAACTTTCTCGAGAAAGTTATGTTTAGGATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATCCTAAACATAACTTTCTCGAGAAAGTTATGTTTAGGATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000285945