Construct: shRNA TRCN0000176640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153152.2-321s1c1
DNA Barcode:
CCTCCAAAGATTAAATTTCCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2410015M20Rik (224904)
Gene Description:
RIKEN cDNA 2410015M20 gene
Transcript:
RefSeq NM_153152.2 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAAGATTAAATTTCCA
Hairpin Sequence:
5'-CCGG-CCTCCAAAGATTAAATTTCCA-CTCGAG-TGGAAATTTAATCTTTGGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAAGATTAAATTTCCACTCGAGTGGAAATTTAATCTTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAAGATTAAATTTCCACTCGAGTGGAAATTTAATCTTTGGAGG-3'

Other clones with same target sequence:

(none)