Construct: shRNA TRCN0000176644

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175393.2-187s1c1
DNA Barcode:
GAAGGAAATTGTTCTGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930555G01Rik (108978)
Gene Description:
RIKEN cDNA 4930555G01 gene
Transcript:
RefSeq NM_175393.2 (NON-CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176644)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGAAATTGTTCTGGAGAA
Hairpin Sequence:
5'-CCGG-GAAGGAAATTGTTCTGGAGAA-CTCGAG-TTCTCCAGAACAATTTCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGAAATTGTTCTGGAGAACTCGAGTTCTCCAGAACAATTTCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGAAATTGTTCTGGAGAACTCGAGTTCTCCAGAACAATTTCCTTC-3'

Other clones with same target sequence:

(none)