Construct: shRNA TRCN0000176651

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183219.1-1306s1c1
DNA Barcode:
CGTTCCTGATAAGTGAATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B230218O03 (329333)
Gene Description:
hypothetical protein B230218O03
Transcript:
RefSeq NM_183219.1 (NON-CURRENT)
Match location:
Position 1306 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176651)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCCTGATAAGTGAATAAA
Hairpin Sequence:
5'-CCGG-CGTTCCTGATAAGTGAATAAA-CTCGAG-TTTATTCACTTATCAGGAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCCTGATAAGTGAATAAACTCGAGTTTATTCACTTATCAGGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCCTGATAAGTGAATAAACTCGAGTTTATTCACTTATCAGGAACG-3'

Other clones with same target sequence:

(none)