Construct: shRNA TRCN0000176688

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020575.1-2256s1c1
DNA Barcode:
CCATAAGAGAATGCTTGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
March7 (57438)
Gene Description:
membrane-associated ring finger (C3HC4) 7
Transcript:
RefSeq NM_020575.1 (NON-CURRENT)
Match location:
Position 2256 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176688)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAAGAGAATGCTTGATAT
Hairpin Sequence:
5'-CCGG-CCATAAGAGAATGCTTGATAT-CTCGAG-ATATCAAGCATTCTCTTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAAGAGAATGCTTGATATCTCGAGATATCAAGCATTCTCTTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAAGAGAATGCTTGATATCTCGAGATATCAAGCATTCTCTTATGG-3'

Other clones with same target sequence:

(none)