Construct: shRNA TRCN0000176696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027569.1-4186s1c1
DNA Barcode:
CCACATAATAATGTGAGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spag9 (70834)
Gene Description:
sperm associated antigen 9
Transcript:
RefSeq NM_027569.1 (NON-CURRENT)
Match location:
Position 4186 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATAATAATGTGAGCATT
Hairpin Sequence:
5'-CCGG-CCACATAATAATGTGAGCATT-CTCGAG-AATGCTCACATTATTATGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATAATAATGTGAGCATTCTCGAGAATGCTCACATTATTATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATAATAATGTGAGCATTCTCGAGAATGCTCACATTATTATGTGG-3'

Other clones with same target sequence:

(none)