Construct: shRNA TRCN0000176700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146097.1-611s1c1
DNA Barcode:
CAGACATGATTCTCATCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbwd1 (226043)
Gene Description:
COBW domain containing 1
Transcript:
RefSeq NM_146097.1 (NON-CURRENT)
Match location:
Position 611 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACATGATTCTCATCAATA
Hairpin Sequence:
5'-CCGG-CAGACATGATTCTCATCAATA-CTCGAG-TATTGATGAGAATCATGTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACATGATTCTCATCAATACTCGAGTATTGATGAGAATCATGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACATGATTCTCATCAATACTCGAGTATTGATGAGAATCATGTCTG-3'

Other clones with same target sequence:

(none)