Construct: shRNA TRCN0000176743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178767.2-537s1c1
DNA Barcode:
CCTTGAAGTGACCAGTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agmo (319660)
Gene Description:
alkylglycerol monooxygenase
Transcript:
RefSeq NM_178767.2 (NON-CURRENT)
Match location:
Position 537 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGAAGTGACCAGTTATAT
Hairpin Sequence:
5'-CCGG-CCTTGAAGTGACCAGTTATAT-CTCGAG-ATATAACTGGTCACTTCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGAAGTGACCAGTTATATCTCGAGATATAACTGGTCACTTCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGAAGTGACCAGTTATATCTCGAGATATAACTGGTCACTTCAAGG-3'

Other clones with same target sequence:

(none)