Construct: shRNA TRCN0000176747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019457.1-1694s1c1
DNA Barcode:
CTATAATTGAACTTCGAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc6 (54562)
Gene Description:
leucine rich repeat containing 6 (testis)
Transcript:
RefSeq NM_019457.1 (NON-CURRENT)
Match location:
Position 1694 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATAATTGAACTTCGAGCTT
Hairpin Sequence:
5'-CCGG-CTATAATTGAACTTCGAGCTT-CTCGAG-AAGCTCGAAGTTCAATTATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATAATTGAACTTCGAGCTTCTCGAGAAGCTCGAAGTTCAATTATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATAATTGAACTTCGAGCTTCTCGAGAAGCTCGAAGTTCAATTATAG-3'

Other clones with same target sequence:

(none)