Construct: shRNA TRCN0000176799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201530.1-95s1c1
DNA Barcode:
CCAAAGGAAGGTTACTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sly (382301)
Gene Description:
Sycp3 like Y-linked
Transcript:
RefSeq NM_201530.1 (NON-CURRENT)
Match location:
Position 95 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGGAAGGTTACTTATTA
Hairpin Sequence:
5'-CCGG-CCAAAGGAAGGTTACTTATTA-CTCGAG-TAATAAGTAACCTTCCTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGGAAGGTTACTTATTACTCGAGTAATAAGTAACCTTCCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGGAAGGTTACTTATTACTCGAGTAATAAGTAACCTTCCTTTGG-3'

Other clones with same target sequence:

(none)