Construct: shRNA TRCN0000176804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019457.1-1818s1c1
DNA Barcode:
GCAAATTGTTCAAACTCAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lrrc6 (54562)
Gene Description:
leucine rich repeat containing 6 (testis)
Transcript:
RefSeq NM_019457.1 (NON-CURRENT)
Match location:
Position 1818 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAATTGTTCAAACTCAGAA
Hairpin Sequence:
5'-CCGG-GCAAATTGTTCAAACTCAGAA-CTCGAG-TTCTGAGTTTGAACAATTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAATTGTTCAAACTCAGAACTCGAGTTCTGAGTTTGAACAATTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAATTGTTCAAACTCAGAACTCGAGTTCTGAGTTTGAACAATTTGC-3'

Other clones with same target sequence:

(none)