Construct: shRNA TRCN0000176953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199012.1-4000s1c1
DNA Barcode:
GCTGATCTTCAAAGTGCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fchsd2 (207278)
Gene Description:
FCH and double SH3 domains 2
Transcript:
RefSeq NM_199012.1 (NON-CURRENT)
Match location:
Position 4000 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATCTTCAAAGTGCTTAT
Hairpin Sequence:
5'-CCGG-GCTGATCTTCAAAGTGCTTAT-CTCGAG-ATAAGCACTTTGAAGATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATCTTCAAAGTGCTTATCTCGAGATAAGCACTTTGAAGATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATCTTCAAAGTGCTTATCTCGAGATAAGCACTTTGAAGATCAGC-3'

Other clones with same target sequence:

(none)