Construct: shRNA TRCN0000176973

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029131.1-172s1c1
DNA Barcode:
GAATGAAATCATTCTGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930503E14Rik (74954)
Gene Description:
RIKEN cDNA 4930503E14 gene
Transcript:
RefSeq NM_029131.1 (NON-CURRENT)
Match location:
Position 172 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000176973)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGAAATCATTCTGGGAAA
Hairpin Sequence:
5'-CCGG-GAATGAAATCATTCTGGGAAA-CTCGAG-TTTCCCAGAATGATTTCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGAAATCATTCTGGGAAACTCGAGTTTCCCAGAATGATTTCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGAAATCATTCTGGGAAACTCGAGTTTCCCAGAATGATTTCATTC-3'

Other clones with same target sequence:

(none)