Construct: shRNA TRCN0000177000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007996.1-413s1c1
DNA Barcode:
GCTCTACTTGTCATCTTATCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fdx1 (14148)
Gene Description:
ferredoxin 1
Transcript:
RefSeq NM_007996.1 (NON-CURRENT)
Match location:
Position 413 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTACTTGTCATCTTATCT
Hairpin Sequence:
5'-CCGG-GCTCTACTTGTCATCTTATCT-CTCGAG-AGATAAGATGACAAGTAGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTACTTGTCATCTTATCTCTCGAGAGATAAGATGACAAGTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTACTTGTCATCTTATCTCTCGAGAGATAAGATGACAAGTAGAGC-3'

Other clones with same target sequence:

(none)