Construct: shRNA TRCN0000177015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172474.1-652s1c1
DNA Barcode:
GAAACCATTTCAAACTCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tyw3 (209584)
Gene Description:
tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
Transcript:
RefSeq NM_172474.1 (NON-CURRENT)
Match location:
Position 652 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000177015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCATTTCAAACTCACAT
Hairpin Sequence:
5'-CCGG-GAAACCATTTCAAACTCACAT-CTCGAG-ATGTGAGTTTGAAATGGTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCATTTCAAACTCACATCTCGAGATGTGAGTTTGAAATGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCATTTCAAACTCACATCTCGAGATGTGAGTTTGAAATGGTTTC-3'

Other clones with same target sequence:

(none)